Plug in and power on the spectrophotometer. Then, find the wavelength knob beside the sample compartment and rotate it to set the wavelength. Manufacturers using a spectrophotometer benefit from higher product quality and consistency, increasing customer satisfaction. Turn the Device on and. Spectrophotometry is an experimental technique that is used to measure the concentration of solutes in a specific solution by calculating the amount of light absorbed by those solutes. This technique is powerful because certain compounds will absorb different wavelengths of light at different. A spectrometer is an analytical tool used across various scientific disciplines to measure how a substance interacts with light. Whether you're a student, researcher, or lab enthusiast, this tutorial will help you ma.